Título: "In -planta" penetration and translocation of Fe@C magnetic nanoparticles
Autor: Marquina, Clara; Corredor, Eduardo; Fuente, Jesús M. de la; Rubiales, Diego; Testillano, P.S.
Resumen: Magnetic
nanoparticles
have
been
implemented
in
biotechnological
and
biomedical
applications
related
to
animal
(in
particular
human)
cells
and
pathologies.
However,
their
use
in
plant
research
biology
is
far
less
studied,
although
it
could
lead
to
the
development
of
interesting
plant
biotechnology
applications.
For
this
purpose
the
first
stage
is
to
work
out
the
penetration
and
transport
of
the
magnetic
nanoparticles
into
living
plants
and
plant
cells
and
how
they
interact
with
plant
cells
and
tissues
[1].
We
have
synthesized
biocompatible
suspensions
of
iron-‐oxide
carbon-‐coated
nanoparticles,
obtained
by
a
gas-‐phase
condensation
method.
They
have
been
injected
into
pumpkin
(Cucurbita
pepo)
living
plants.
The
graphitic
shell
of
our
nanoparticles
made
possible
their
visualization
into
plant
cells
and
tissues,
using
different
microscopy
techniques
(fluorescence,
confocal,
light
and
electron
microscopy).
Moreover,
their
magnetic
character
allowed
the
nanoparticles
to
be
positioned
in
the
desired
plant
tissue
by
applying
magnetic
field
gradients.
In
the
absence
of
magnetic
fields,
the
nanoparticles
can
as
well
travel
along
the
vascular
system,
reaching
different
cell
and
tissues.
Nanoparticles
have
been
found
both
in
the
cytoplasm
and
in
the
extracellular
space
between
cells.
A
size-‐based
selection
mechanism
seems
to
be
operating,
probably
involving
cell
walls
and
waxes
acting
as
a
barrier.
Accumulation
of
nanoparticles
was
detected
in
leaf
trichomes,
suggesting
a
way
for
nanoparticles
excretion/detoxification.
With
respect
to
cytotoxicity,
it
has
been
observed
that
cells
containing
nanoparticle
agglomerates
exhibited
a
cytoplasm
denser
than
that
of
cells
containing
few
nanoparticles.
Damage
at
the
plant
level
was
not
macroscopically
evident.
Although
more
studies
are
necessary
to
unveil
the
nanoparticles
penetration
and
translocation
mechanisms
as
well
as
their
cyto-‐
and
phyto-‐toxicity,
our
results
open
a
wide
range
of
possibilities
for
using
magnetic
nanoparticles
in
general
plant
research
and
agronomy.
Descripción: Trabajo presentado en la 20th International Conference of Magnetism, celebrada en Barcelona del 5 al 10 de julio de 2015.-- Marquina, Clara et al.
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