Título: Interaction of superparamagnetic nanoparticles and quantum dots with pathogenic fungi: internalization and toxicity profile
Autor: Rispail, Nicolas; Testillano, P.S.; Risueño, María Carmen; Rubiales, Diego; Prats, Elena
Resumen: For several
years
now,
nanoscaled
materials
have
been
implemented
in
biotechnological
applications
related
to
animal
(in
particular
human)
cells
and
related
pathologies.
However,
the
use
of
nanomaterials
in
plant
biology
is
far
less
widespread,
although
their
application
in
this
field
could
lead
to
the
future
development
of
plant
biotechnology
applications.
For
any
practical
use,
it
is
crucial
to
elucidate
the
relationship
between
the
nanomaterials
and
the
target
cells.
In
this
work
we
have
evaluated
the
behavior
of
two
types
of
nanomaterials,
superparamagnetic
nanoparticles
and
quantum
dots,
on
Fusarium
oxysporum,
a
fungal
species
that
infects
an
enormous
range
of
crops
causing
important
economic
losses
and
is
also
an
opportunistic
human
pathogen
[1].
Our
results
indicated
that
both
nanomaterials
rapidly
interacted
with
the
fungal
hypha
labeling
the
presence
of
the
pathogenic
fungus,
although
they
showed
differential
behavior
with
respect
to
internalization.
Thus,
whereas
magnetic
nanoparticles
appeared
to
be
on
the
cell
surface,
quantum
dots
were
significantly
taken
up
by
the
fungal
hyphae
showing
their
potential
for
the
development
of
novel
control
approaches
of
F.
oxysporum
and
related
pathogenic
fungi
following
appropriate
functionalization.
In
addition,
the
fungal
germination
and
growth,
accumulation
of
ROS,
indicative
of
cell
stress,
and
fungal
viability
have
been
evaluated
at
different
nanomaterial
concentrations
showing
the
low
toxicity
of
both
types
of
nanomaterials
to
the
fungus.
This
work
represents
the
first
study
on
the
behavior
of
quantum
dots
and
superparamagnetic
particles
on
fungal
cells,
and
constitutes
the
first
and
essential
step
to
address
the
feasibility
of
new
nanotechnology-‐based
systems
for
early
detection
and
eventual
control
of
pathogenic
fungi.
Descripción: Trabajo presentado en la 20th International Conference of Magnetism, celebrada en Barcelona del 5 al 10 de julio de 2015. -- Rispail, Nicolas et al.
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