Título: Field-responsive fluids of carbon-coated magnetic nanoparticles for plant biology and agronomy
Autor: Cifuentes, Zuny; Coronado, María José; Corredor, Eduardo; Custardoy, Laura; Fernández-Pacheco, Rodrigo; Fuente, Jesús M. de la; González-Melendi, Pablo; Ibarra, M. Ricardo; Marquina, Clara; Pérez de Luque, Alejandro; Risueño, María Carmen; Rubiales, Diego; Testillano, P.S.
Resumen: The ongoing research
on magnetic
nanoparticles
(MNPs)
for bio-medical
applications
and
the
achievements related
to
their use in diagnosis and
therapy
open
a wide range of possibilities for using
MNPs
in
other
disciplines, such as
general plant research and agronomy. For
this purpose,
the first stage is to work out
the penetration and transport into living
plants,
and how inorganic nanoparticles
interact with plant cells and tissues1.
We
have used
biocompatible suspensions
of
iron-oxide
carbon-coated nanoparticles,
synthesized by a gas-phase condensation
method.
They
have been injected into
pumpkin (Cucurbita pepo) living plants.
The graphitic shell of our nanoparticles
has
made possible their visualization into
plant cells and tissues,
by
microscopy
techniques (fluorescence, confocal, light
and electron microscopy). Moreover,
their magnetic character
has
allowed the
MNPs
to be positioned in the desired
plant tissue by applying magnetic field
gradients. We have observed that the
nanoparticles can travel along the
vascular system, reaching different cell
and tissues.
They
have been found both
in the cytoplasm and in the extracellular
space between cells. A size-based
selection mechanism seems to be
operating, probably involving cell walls
and
waxes acting as a barrier.
Accumulation of
MNPs
has been
detected in leaf trichomes, suggesting a
way for nanoparticle
excretion
and
detoxification. Cyto-and phyto-toxicity
have
been
as well evaluated.
Although
more studies are necessary,
our results
open a wide range of possibilities for
using magnetic nanoparticles in general
plant research and agronomy.
Descripción: Trabajo presentado en el 14th International Conference on Electrorheological Fluids and Magnetorheological Suspensions (ERMR 2014), celebrado en Granada del 7 al 11 de julio de 2014.
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